Chlorogenic acid regulates the expression of protein phosphatase 2A subunit B in the cerebral cortex of a rat stroke model and glutamate-exposed neurons

Chlorogenic acid regulates the expression of protein phosphatase 2A subunit B in the cerebral cortex of a rat stroke model and glutamate-exposed neurons

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Abstract Background Ischemic stroke is a serious neurological disorder caused by blockages in cerebral artery.Protein phosphatase 2A (PP2A) is a phosphatase that performs a critical role in cell signaling and growth.PP2A subunit B acts as a neuroprotective agent in the nerve system.Chlorogenic acid, which is mainly found in roasted coffee, has antioxidant, anti-inflammatory, and anti-apoptotic effects.We hypothesized that chlorogenic acid modulates PP2A subunit B expression in ischemic stroke models and glutamate-mediated neurons.

Middle artery occlusion (MCAO) surgery was operated and chlorogenic Equipment acid (30 mg/kg) or phosphate buffer saline was treated 2 h after MCAO.The cerebral cortex was collected 24 h after surgery and the change of PP2A subunit B expression was analyzed.Glutamate and/or chlorogenic acid were treated in cultured neurons, further study was performed.Results A decrease in PP2A subunit B expression in MCAO animals was identified.Chlorogenic acid alleviated this decrease due to ischemic injury.

Moreover, the number of PP2A subunit B-positive cells in the ischemic cerebral cortex was significantly decreased, chlorogenic acid alleviated this decrease.We also found protective effects of chlorogenic Faber Cooker Hood Grèxia 330.0543.464 220 - 240 V gres dk bk a60 p1705 acid in neurons exposed to glutamate.Glutamate decreased the expression of PP2A subunit B and chlorogenic acid mitigated this decrease.Our results elucidated that chlorogenic acid performs neuroprotective functions and attenuates the reduction of PP2A subunit B by brain damage and glutamate-mediated excitotoxicity.Conclusions We showed that chlorogenic acid attenuated the decrease of PP2A subunit B in ischemic injury and neurons exposed to glutamate.

Since PP2A subunit B contributes to the protection of brain tissue, we can suggest that chlorogenic acid preserves neurons by modulating PP2A subunit B during ischemic damage.